Purification of Limulus polyphemus proclotting enzyme.
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چکیده
منابع مشابه
Purification of Limulus polyphemus proclotting enzyme.
Horseshoe crabs (Limulus polyphemus and Tachypleus tridentatus) possess a proteolytic blood coagulation system within their amebocytes that, after release and endotoxin activation, generates a polymerized insoluble coagulin clot. Clotting enzyme from horseshoe crab amebocyte lysate is the protease that activates the clottable protein (coagulogen) which then forms the coagulin clot. Comparison o...
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متن کاملIsolation, purification and characterization of an iron-binding protein from the horseshoe crab (Limulus polyphemus).
The presence of an iron-binding protein in the haemolymph of the horseshoe crab, Limulus polyphemus, was detected by gel filtration of 59Fe-labelled haemolymph. Lysis of amoebocytes did not change the amount of iron-binding protein in haemolymph samples. The protein was purified to homogeneity by ion-exchange chromatography. The molecular mass of the purified protein was estimated to be 282,000...
متن کاملEffects of blood extraction on horseshoe crabs (Limulus polyphemus)
E-mail address (for J. Berkson, contact author): [email protected] the United States, mandated that all biomedical companies actively bleed ing horseshoe crabs estimate mortal ity rates resulting from their bleeding process (Schrading et al.3). Because of the unique methods of the different biomedical companies, each company was required to quantify its own rate of mortality. BioWhittaker, a CA...
متن کاملThe pace Maker of the Cardiac Ganglion of Limulus .polyphemus
The ganglion on the dorsal surface of the heart of Limulus polyphemus originates the impulses which cause the contractions of the cardiac muscle (1). In large specimens the portion of the dorsal nerve cord which contains the ganglion cells may be 10 or even 12 cm. in length and one naturally wonders where in the extent of this long structure the impulse actually arises. Does it arise in a speci...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1992
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)35951-9